Taq DNA Polymerase    is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´-->3´ direction in the presence of magnesium and also possesses a 5´-->3´ exonuclease activity.

 Pfu DNA Polymerase  is a thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus DSM3638. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´-->3´ direction in the presence of magnesium.